Hotspot

rpl2-trnl-CAU

LSC · 89418-89944 · 527 bp

Back to Neolitsea

Pi 0.00367

MarkerSeek score 59.20

Primer available yes

Label rpl2-trnl-CAU

Feature Metadata

label_name: rpl2-trnl-CAU
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 4
pi: 0.00367
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnl-CAU_p1	TCGCGGATTGGATTTCACTT	GAATGGTTAAAGCGCCCAAC	45.0	50.0	57.9	58.0	645-692 bp	1.0	82.7
rpl2-trnl-CAU_p2	TTCGCGGATTGGATTTCACT	GAATGGTTAAAGCGCCCAAC	45.0	50.0	57.9	58.0	646-693 bp	1.0	82.7
rpl2-trnl-CAU_p3	TCGCGGATTGGATTTCACTT	TGAATTTGGGTCACTTGCGA	45.0	45.0	57.9	58.0	697-744 bp	1.0	82.3
rpl2-trnl-CAU_p4	TTCGCGGATTGGATTTCACT	TGAATTTGGGTCACTTGCGA	45.0	45.0	57.9	58.0	698-745 bp	1.0	82.3
rpl2-trnl-CAU_p5	CGCGGATTGGATTTCACTTG	GAATGGTTAAAGCGCCCAAC	50.0	50.0	57.8	58.0	644-691 bp	1.0	82.1