Hotspot

psaJ-rpl33

LSC · 70931-71358 · 428 bp

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Pi 0.0039

MarkerSeek score 58.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 5
pi: 0.0039
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	AATGCCCCGCGATTCTTTAT	45.0	45.0	58.1	58.0	549-550 bp	1.0	80.1
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	ATGCCCCGCGATTCTTTATT	45.0	45.0	58.1	58.0	548-549 bp	1.0	80.1
psaJ-rpl33_p3	TTCCCAGATGCGTTGACATT	AATGCCCCGCGATTCTTTAT	45.0	45.0	57.8	58.0	556-557 bp	1.0	79.1
psaJ-rpl33_p4	TTCCCAGATGCGTTGACATT	ATGCCCCGCGATTCTTTATT	45.0	45.0	57.8	58.0	555-556 bp	1.0	79.1
psaJ-rpl33_p5	TCCCAGATGCGTTGACATTC	ATGCCCCGCGATTCTTTATT	50.0	45.0	58.3	58.0	554-555 bp	1.0	78.6