Hotspot

ycf3-trnS-GGA

LSC · 46728-47542 · 815 bp

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Pi 0.00082
MarkerSeek score 59.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
815
variable_sites
2
pi
0.00082
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCTCCTGAGGTTGTCGGAAT TTGTGGTCGGTTGGTGAATT 50.0 45.0 58.1 57.9 1024-1028 bp 1.0 79.5
ycf3-trnS-GGA_p2 TCTCCTGAGGTTGTCGGAAT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.1 58.2 994-998 bp 1.0 78.8
ycf3-trnS-GGA_p3 TCTCCTGAGGTTGTCGGAAT TTCCAATGCTACGCCTTGAA 50.0 45.0 58.1 57.8 944-948 bp 1.0 78.5
ycf3-trnS-GGA_p4 TCTCCTGAGGTTGTCGGAAT ATTGTGGTCGGTTGGTGAAT 50.0 45.0 58.1 57.7 1025-1029 bp 1.0 77.7
ycf3-trnS-GGA_p5 TTCTCCTGAGGTTGTCGGAA TTGTGGTCGGTTGGTGAATT 50.0 45.0 58.3 57.9 1025-1029 bp 1.0 77.7