Hotspot

ycf1

SSC · 124871-130501 · 5631 bp

Back to Neolamarckia

Pi 0.00534

MarkerSeek score 42.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5631
variable_sites: 30
pi: 0.00534
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTCGGCCTCTTTTCCTACC	CTATGCGCTCTCAAAGACGT	50.0	50.0	57.3	58.1	165-174 bp	1.0	79.0
ycf1_p2	ATTCGGCCTCTTTTCCTACC	TGCGCTCTCAAAGACGTAAA	50.0	45.0	57.3	57.9	162-171 bp	1.0	78.6
ycf1_p3	ATTCGGCCTCTTTTCCTACC	TCTATGCGCTCTCAAAGACG	50.0	50.0	57.3	57.8	166-175 bp	1.0	78.2
ycf1_p4	ATTCGGCCTCTTTTCCTACC	CCTCCGGATCAAACAAACGA	50.0	50.0	57.3	58.2	244-253 bp	1.0	76.6
ycf1_p5	ATTCGGCCTCTTTTCCTACC	TCCTCCGGATCAAACAAACG	50.0	50.0	57.3	58.2	245-254 bp	1.0	76.6