Hotspot

rps16

LSC · 5069-6154 · 1086 bp

Back to Neolamarckia

Pi 0.0111

MarkerSeek score 60.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1086
variable_sites: 12
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGATAAATAGATAGGGGAGGGGG	TCAATTCCATACCGGTTCCA	47.8	45.0	57.8	56.5	1219-1225 bp	1.0	43.1
rps16_p2	AGATAAATAGATAGGGGAGGGGG	TCAATTCCATACCGGTTCCAA	47.8	42.9	57.8	57.3	1219-1225 bp	1.0	41.1
rps16_p3	TAAATAGATAGGGGAGGGGG	TCAATTCCATACCGGTTCCA	50.0	45.0	54.4	56.5	1216-1222 bp	1.0	40.9
rps16_p4	AGATAAATAGATAGGGGAGGGGG	ACCGGTTCCAAATTAGAGGA	47.8	45.0	57.8	56.1	1209-1215 bp	1.0	40.9
rps16_p5	GATAAATAGATAGGGGAGGGGG	TCAATTCCATACCGGTTCCA	50.0	45.0	56.3	56.5	1218-1224 bp	1.0	40.9