Hotspot

accD

LSC · 58882-60501 · 1620 bp

Back to Neolamarckia

Pi 0.00319

MarkerSeek score 63.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1620
variable_sites: 5
pi: 0.00319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAATATTCAAACTCCTTACCT	TCCGCAACTTTTGATTCTTTCT	31.8	36.4	52.5	56.8	1795-1849 bp	1.0	42.6
accD_p2	TCAATATTCAAACTCCTTACCTT	TCCGCAACTTTTGATTCTTTCT	30.4	36.4	53.4	56.8	1795-1849 bp	1.0	42.6
accD_p3	TTCAATATTCAAACTCCTTACCT	TCCGCAACTTTTGATTCTTTCT	30.4	36.4	53.4	56.8	1796-1850 bp	1.0	42.6
accD_p4	TTCAATATTCAAACTCCTTACCTT	TCCGCAACTTTTGATTCTTTCT	29.2	36.4	54.2	56.8	1796-1850 bp	1.0	42.6
accD_p5	TTTCAATATTCAAACTCCTTACCT	TCCGCAACTTTTGATTCTTTCT	29.2	36.4	54.2	56.8	1797-1851 bp	1.0	42.6