Hotspot

ndhI-ndhA

SSC · 120678-120764 · 87 bp

Back to Neolamarckia

Pi 0.0465
MarkerSeek score 68.50
Primer available yes
Label ndhI-ndhA

Feature Metadata

label_name
ndhI-ndhA
feature_type
igs
region
SSC
length_bp
87
variable_sites
4
pi
0.0465
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhA_p1 ACTCAGTTACCATAGGGAACA CAAGGCTGAGAATGGACCAA 42.9 50.0 55.7 57.8 215-216 bp 1.0 53.8
ndhI-ndhA_p2 ACTCAGTTACCATAGGGAACA AAGGCTGAGAATGGACCAAC 42.9 50.0 55.7 57.8 214-215 bp 1.0 53.8
ndhI-ndhA_p3 GAACTCAGTTACCATAGGGAACA CAAGGCTGAGAATGGACCAA 43.5 50.0 57.6 57.8 217-218 bp 1.0 52.9
ndhI-ndhA_p4 TGAACTCAGTTACCATAGGGAAC CAAGGCTGAGAATGGACCAA 43.5 50.0 57.6 57.8 218-219 bp 1.0 52.9
ndhI-ndhA_p5 GAACTCAGTTACCATAGGGAACA AAGGCTGAGAATGGACCAAC 43.5 50.0 57.6 57.8 216-217 bp 1.0 52.9