Hotspot

ndhC-trnV-UAC

LSC · 51804-52992 · 1189 bp

Back to Neolamarckia

Pi 0.0085

MarkerSeek score 64.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1189
variable_sites: 10
pi: 0.0085
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1320-1322 bp	1.0	78.7
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1332-1334 bp	1.0	77.1
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1339-1341 bp	1.0	76.4
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1319-1321 bp	1.0	76.0
ndhC-trnV-UAC_p5	TCCTTTGCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	1333-1335 bp	1.0	75.8