Hotspot

ycf3-trnS-GGA

LSC · 45874-46275 · 402 bp

Back to Neolamarckia

Pi 0.00995

MarkerSeek score 54.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 4
pi: 0.00995
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	AATGCCTCCCTTTCTCCTGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	597 bp	1.0	76.7
ycf3-trnS-GGA_p2	ATGCCTCCCTTTCTCCTGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	596 bp	1.0	76.7
ycf3-trnS-GGA_p3	AATGCCTCCCTTTCTCCTGA	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.3	57.8	547 bp	1.0	76.4
ycf3-trnS-GGA_p4	ATGCCTCCCTTTCTCCTGAA	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.3	57.8	546 bp	1.0	76.4
ycf3-trnS-GGA_p5	ATGCCTCCCTTTCTCCTGAA	GGATTCGAACCCTCGGTAAG	50.0	55.0	58.3	57.8	584 bp	1.0	76.2