Hotspot

trnC-GCA-petN

LSC · 28545-29395 · 851 bp

Back to Neolamarckia

Pi 0.0153

MarkerSeek score 65.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 13
pi: 0.0153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGTATATCGTTTTGGCGGCA	ACTTCTTCCCCACACTACGA	45.0	50.0	58.0	58.0	1016-1037 bp	1.0	80.8
trnC-GCA-petN_p2	TGTATATCGTTTTGGCGGCA	TTCTTCCCCACACTACGAGT	45.0	50.0	58.0	58.0	1014-1035 bp	1.0	80.8
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	961-982 bp	1.0	80.4
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	959-980 bp	1.0	80.4
trnC-GCA-petN_p5	TGTATATCGTTTTGGCGGCA	CACTTCTTCCCCACACTACG	45.0	55.0	58.0	57.9	1017-1038 bp	1.0	80.2