Hotspot

trnH-GUG-psbA

LSC · 92-390 · 299 bp

Back to Neolamarckia

Pi 0.0558

MarkerSeek score 75.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 299
variable_sites: 15
pi: 0.0558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	361-376 bp	1.0	85.6
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	362-377 bp	1.0	85.4
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	354-369 bp	1.0	84.1
trnH-GUG-psbA_p4	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.5	57.9	383-398 bp	1.0	82.6
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	TCCCTCTAGACCTAGCTGCT	50.0	55.0	57.9	58.8	360-375 bp	1.0	80.0