Hotspot

rpl16

LSC · 81016-82594 · 1579 bp

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Pi 0.00322

MarkerSeek score 60.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1579
variable_sites: 5
pi: 0.00322
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGTCCAGAGATAAAGAAATCCGT	TCAAAGAAAAACGAACAATTAGA	39.1	26.1	57.3	53.2	1730-1745 bp	1.0	41.4
rpl16_p2	TGTCCAGAGATAAAGAAATCCGT	TCTTAATCAAAGAAAAACGAACA	39.1	26.1	57.3	53.2	1736-1751 bp	1.0	41.4
rpl16_p3	TGTCCAGAGATAAAGAAATCCGT	TCAAAGAAAAACGAACAATTAGAA	39.1	25.0	57.3	54.0	1730-1745 bp	1.0	41.4
rpl16_p4	TGTCCAGAGATAAAGAAATCCGT	TCTTAATCAAAGAAAAACGAACAA	39.1	25.0	57.3	54.0	1736-1751 bp	1.0	41.4
rpl16_p5	TTGTCCAGAGATAAAGAAATCCGT	TCAAAGAAAAACGAACAATTAGA	37.5	26.1	58.0	53.2	1731-1746 bp	1.0	41.4