Hotspot

clpP

LSC · 68713-71002 · 2290 bp

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Pi 0.00393

MarkerSeek score 53.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2290
variable_sites: 9
pi: 0.00393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATAGAGCGGGTTGGCTTAGA	TGAAGACAATAAAACCATTGTTACGT	50.0	30.8	58.2	58.1	2471-2473 bp	1.0	40.4
clpP_p2	TAGAGCGGGTTGGCTTAGAT	TGAAGACAATAAAACCATTGTTACGT	50.0	30.8	58.2	58.1	2470-2472 bp	1.0	40.4
clpP_p3	CGGGTTGGCTTAGATCGATC	TGAAGACAATAAAACCATTGTTACGT	55.0	30.8	58.2	58.1	2465-2467 bp	1.0	40.4
clpP_p4	ATAGAGCGGGTTGGCTTAGA	AGACAATAAAACCATTGTTACGT	50.0	30.4	58.2	54.8	2468-2470 bp	1.0	40.4
clpP_p5	TAGAGCGGGTTGGCTTAGAT	AGACAATAAAACCATTGTTACGT	50.0	30.4	58.2	54.8	2467-2469 bp	1.0	40.4