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clpP-psbB

LSC · 71003-71807 · 805 bp

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Pi 0.0109

MarkerSeek score 66.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 8
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	58.0	58.0	954-979 bp	1.0	84.4
clpP-psbB_p2	CTTTTGGAACACCAATGGGC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	900-925 bp	1.0	83.1
clpP-psbB_p3	ACCCAAACTGCATCTTCCTC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	940-965 bp	1.0	82.8
clpP-psbB_p4	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	876-901 bp	1.0	82.8
clpP-psbB_p5	TCCTCTCCAGGATTCCGAAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	925-950 bp	1.0	82.5