Hotspot

accD-psaI

LSC · 57731-58642 · 912 bp

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Pi 0.00987

MarkerSeek score 64.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 9
pi: 0.00987
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ACGGTTTCCTTCCCTTGAAC	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	1018-1036 bp	1.0	80.5
accD-psaI_p2	CACGGTTTCCTTCCCTTGAA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	1019-1037 bp	1.0	80.5
accD-psaI_p3	TTCGACCCAATTGTACCACG	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.2	58.0	1079-1097 bp	1.0	79.0
accD-psaI_p4	CGACCCAATTGTACCACGTA	AAAGAAGCCATTGCGATTGC	50.0	45.0	57.6	58.0	1077-1095 bp	1.0	77.7
accD-psaI_p5	GGTTTCCTTCCCTTGAACCA	AAAGAAGCCATTGCGATTGC	50.0	45.0	57.6	58.0	1016-1034 bp	1.0	77.7