Hotspot

trnF-GAA-trnV-UAC

LSC · 49478-50034 · 557 bp

Back to Neofinetia

Pi 0.00216

MarkerSeek score 60.60

Primer available yes

Label trnF-GAA-trnV-UAC

Feature Metadata

label_name: trnF-GAA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 1
pi: 0.00216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnV-UAC_p1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	584-624 bp	1.0	82.1
trnF-GAA-trnV-UAC_p2	GGAAATGGTCGGGATAGCTC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.8	57.8	630-670 bp	1.0	81.2
trnF-GAA-trnV-UAC_p3	AATGGTCGGGATAGCTCAGT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	627-667 bp	1.0	80.9
trnF-GAA-trnV-UAC_p4	ATGGTCGGGATAGCTCAGTT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	626-666 bp	1.0	80.9
trnF-GAA-trnV-UAC_p5	CTCTGCTAGGATGATGCACG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	650-690 bp	1.0	80.8