Hotspot

psbK-psbI

LSC · 7610-8100 · 491 bp

Back to Neofinetia

Pi 0.0102

MarkerSeek score 49.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 5
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	887-893 bp	1.0	80.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	888-894 bp	1.0	80.8
psbK-psbI_p3	TCGTTTCTGATGCGAACGAT	TGGACTAAAGCGTCGGATTG	45.0	50.0	58.0	58.0	1507-1513 bp	1.0	80.7
psbK-psbI_p4	TCGTTTCTGATGCGAACGAT	GTGGACTAAAGCGTCGGATT	45.0	50.0	58.0	58.0	1508-1514 bp	1.0	80.7
psbK-psbI_p5	TCGTTTCTGATGCGAACGAT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.0	58.1	1231-1232 bp	1.0	79.9