Hotspot

rps16-trnQ-UUG

LSC · 6073-6979 · 907 bp

Back to Neofinetia

Pi 0.00674

MarkerSeek score 64.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 6
pi: 0.00674
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1003-1007 bp	1.0	80.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	980-984 bp	1.0	80.2
rps16-trnQ-UUG_p3	CCACAGCCAATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1001-1005 bp	1.0	80.0
rps16-trnQ-UUG_p4	ATCCACAGCCAATCATGTCC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	57.9	57.8	1007-1011 bp	1.0	79.6
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	991-995 bp	1.0	79.6