Hotspot

trnK-UUU-rps16

LSC · 4436-4897 · 462 bp

Back to Neofinetia

Pi 0.00866
MarkerSeek score 58.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
462
variable_sites
4
pi
0.00866
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGGATCAGTCGCGGTCTTAT GCTCAACCCACAGAAACTGT 50.0 50.0 58.3 58.3 686-706 bp 1.0 75.5
trnK-UUU-rps16_p2 AGGATCAGTCGCGGTCTTAT GTGCTCAACCCACAGAAACT 50.0 50.0 58.3 58.3 688-708 bp 1.0 75.5
trnK-UUU-rps16_p3 AGGATCAGTCGCGGTCTTAT TGCTCAACCCACAGAAACTG 50.0 50.0 58.3 58.3 687-707 bp 1.0 75.4
trnK-UUU-rps16_p4 GTCGCAATTAAAAGCCGAGT GCTCAACCCACAGAAACTGT 45.0 50.0 57.1 58.3 611-631 bp 1.0 70.8
trnK-UUU-rps16_p5 GTCGCAATTAAAAGCCGAGT GTGCTCAACCCACAGAAACT 45.0 50.0 57.1 58.3 613-633 bp 1.0 70.8