Hotspot

ycf1

IRb · 111227-112165 · 939 bp

Back to Neocinnamomum

Pi 0

MarkerSeek score 25.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 939
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	GACGGAATTACCAACGGTGT	55.0	50.0	58.0	58.2	1591-1609 bp	1.0	80.0
ycf1_p2	CCGGAACTTCTTCGTAGTGG	GACGGAATTACCAACGGTGT	55.0	50.0	58.0	58.2	1590-1608 bp	1.0	80.0
ycf1_p3	CCCGGAACTTCTTCGTAGTG	TGACGGAATTACCAACGGTG	55.0	50.0	58.0	58.2	1592-1610 bp	1.0	80.0
ycf1_p4	CCGGAACTTCTTCGTAGTGG	TGACGGAATTACCAACGGTG	55.0	50.0	58.0	58.2	1591-1609 bp	1.0	80.0
ycf1_p5	CCGGAACTTCTTCGTAGTGG	ACGGAATTACCAACGGTGTT	55.0	45.0	58.0	57.7	1589-1607 bp	1.0	79.5