Hotspot

rpoC1

LSC · 20448-23228 · 2781 bp

Back to Neocinnamomum

Pi 0.000962

MarkerSeek score 57.10

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2781
variable_sites: 5
pi: 0.000962
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCCAGTTGAACCGGAGAGAT	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.1	58.0	2943-2954 bp	1.0	79.6
rpoC1_p2	TCCAGTTGAACCGGAGAGAT	ACGATCTTTGGCTCTGGAAC	50.0	50.0	58.1	57.9	2926-2937 bp	1.0	79.1
rpoC1_p3	CCCCCTTTACTGGATCTCGA	ACGATCTTTGGCTCTGGAAC	55.0	50.0	58.2	57.9	2973-2984 bp	1.0	77.9
rpoC1_p4	TTCCAGTTGAACCGGAGAGA	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.3	58.0	2944-2955 bp	1.0	77.8
rpoC1_p5	CCAGTTGAACCGGAGAGATC	TTGCTCGTTCGAGAACTACG	55.0	50.0	57.7	58.0	2942-2953 bp	1.0	77.7