Hotspot

rpoA

LSC · 79671-80690 · 1020 bp

Back to Neocinnamomum

Pi 0.00196

MarkerSeek score 47.80

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 4
pi: 0.00196
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AAAGAAGAAATAATCCCAATGG	AACGGATGTAGGCCTCCTAA	31.8	50.0	52.4	57.8	1148 bp	1.0	40.3
rpoA_p2	AAAGAAGAAATAATCCCAATGG	ACGGATGTAGGCCTCCTAAA	31.8	50.0	52.4	57.8	1147 bp	1.0	40.3
rpoA_p3	AAAAGAAGAAATAATCCCAATGG	AACGGATGTAGGCCTCCTAA	30.4	50.0	53.2	57.8	1149 bp	1.0	40.3
rpoA_p4	AAAAGAAGAAATAATCCCAATGG	ACGGATGTAGGCCTCCTAAA	30.4	50.0	53.2	57.8	1148 bp	1.0	40.3
rpoA_p5	AAAGAAGAAATAATCCCAATGG	AACCCCTATGCCACATAACG	31.8	50.0	52.4	57.7	1164 bp	1.0	40.3