Hotspot

ndhF-rpl32

SSC · 114614-115583 · 970 bp

Back to Neocinnamomum

Pi 0.00155

MarkerSeek score 61.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 970
variable_sites: 3
pi: 0.00155
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACAAGCATAAGTCCCAACCC	TGCAGCCTGATACCCTTTTC	50.0	50.0	57.8	57.9	1127-1141 bp	1.0	78.5
ndhF-rpl32_p2	ACAAGCATAAGTCCCAACCC	CTGCAGCCTGATACCCTTTT	50.0	50.0	57.8	57.9	1128-1142 bp	1.0	78.5
ndhF-rpl32_p3	GGCACAAGCATAAGTCCCAA	TGCAGCCTGATACCCTTTTC	50.0	50.0	58.5	57.9	1130-1144 bp	1.0	76.6
ndhF-rpl32_p4	GGCACAAGCATAAGTCCCAA	CTGCAGCCTGATACCCTTTT	50.0	50.0	58.5	57.9	1131-1145 bp	1.0	76.5
ndhF-rpl32_p5	CAAGCATAAGTCCCAACCCT	TGCAGCCTGATACCCTTTTC	50.0	50.0	57.5	57.9	1126-1140 bp	1.0	76.1