Hotspot

ycf1-ndhF

SSC · 112166-112381 · 216 bp

Back to Neocinnamomum

Pi 0.0357

MarkerSeek score 79.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 216
variable_sites: 13
pi: 0.0357
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AAGAGGGATCCACCGAAGAA	GGGGCGAATCTCTGCTTATT	50.0	50.0	58.0	57.7	390-408 bp	1.0	84.1
ycf1-ndhF_p2	AAGACCCTTCCCTTTGTTCG	GGGGCGAATCTCTGCTTATT	50.0	50.0	57.7	57.7	372-390 bp	1.0	82.5
ycf1-ndhF_p3	ATCCACCGAAGAAGACCCTT	GGGGCGAATCTCTGCTTATT	50.0	50.0	58.3	57.7	383-401 bp	1.0	81.8
ycf1-ndhF_p4	AGAGGGATCCACCGAAGAAG	GGGGCGAATCTCTGCTTATT	55.0	50.0	58.5	57.7	389-407 bp	1.0	80.5
ycf1-ndhF_p5	GAGGGATCCACCGAAGAAGA	GGGGCGAATCTCTGCTTATT	55.0	50.0	58.5	57.7	388-406 bp	1.0	80.4