Hotspot

psbM-trnD-GUC

LSC · 29338-30326 · 989 bp

Back to Neocinnamomum

Pi 0.00202

MarkerSeek score 60.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 989
variable_sites: 4
pi: 0.00202
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1097-1099 bp	1.0	77.7
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	ACCTCATTTCACCGGGATTG	45.0	50.0	58.3	57.9	1118-1120 bp	1.0	77.3
psbM-trnD-GUC_p3	ATGAACAGCGCAGTAGCAAT	CACCTCATTTCACCGGGATT	45.0	50.0	58.3	57.9	1119-1121 bp	1.0	77.3
psbM-trnD-GUC_p4	ATGAACAGCGCAGTAGCAAT	CCTCATTTCACCGGGATTGT	45.0	50.0	58.3	57.9	1117-1119 bp	1.0	77.3
psbM-trnD-GUC_p5	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1096-1098 bp	1.0	76.7