Hotspot

petN-psbM

LSC · 28157-29232 · 1076 bp

Back to Neocinnamomum

Pi 0.00186

MarkerSeek score 60.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 4
pi: 0.00186
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1240 bp	1.0	77.0
petN-psbM_p2	GGGGACACAATTCACATGGA	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.8	58.3	1262 bp	1.0	76.5
petN-psbM_p3	AGGGGACACAATTCACATGG	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.8	58.3	1263 bp	1.0	76.4
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1241 bp	1.0	76.0
petN-psbM_p5	GGGGACACAATTCACATGGA	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.8	57.6	1263 bp	1.0	75.5