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atpF-atpH

LSC · 12626-13076 · 451 bp

Back to Neocinnamomum

Pi 0.00222

MarkerSeek score 34.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 2
pi: 0.00222
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AGAATCGATTACGTCTTTCA	GATTTACGGACTGGTCGTGG	35.0	55.0	52.1	58.4	526-535 bp	1.0	40.5
atpF-atpH_p2	AAGAATCGATTACGTCTTTCA	GATTTACGGACTGGTCGTGG	33.3	55.0	53.0	58.4	527-536 bp	1.0	40.5
atpF-atpH_p3	AGAATCGATTACGTCTTTCA	CGTGGCATTAGCGCTCTTAT	35.0	50.0	52.1	58.5	511-520 bp	1.0	40.5
atpF-atpH_p4	AAGAATCGATTACGTCTTTCA	CGTGGCATTAGCGCTCTTAT	33.3	50.0	53.0	58.5	512-521 bp	1.0	40.5
atpF-atpH_p5	AAAGAATCGATTACGTCTTTCA	GATTTACGGACTGGTCGTGG	31.8	55.0	53.9	58.4	528-537 bp	1.0	40.5