Hotspot

ycf1

SSC · 125422-130905 · 5484 bp

Back to Nematolepis

Pi 0.0106

MarkerSeek score 44.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5484
variable_sites: 87
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGTTCTCGTTCGTGGTCTG	TACATGGGCCTTATCGTGGA	50.0	50.0	58.2	58.2	224 bp	1.0	80.4
ycf1_p2	TTGTTCTCGTTCGTGGTCTG	GGCCTTATCGTGGAAGACTC	50.0	55.0	58.2	57.8	218 bp	1.0	80.3
ycf1_p3	TTGTTCTCGTTCGTGGTCTG	CATGGGCCTTATCGTGGAAG	50.0	55.0	58.2	58.4	222 bp	1.0	78.8
ycf1_p4	TTGTTCTCGTTCGTGGTCTG	ATGGGCCTTATCGTGGAAGA	50.0	50.0	58.2	58.5	221 bp	1.0	78.1
ycf1_p5	CGATTTGTTCTCGTTCGTGG	TACATGGGCCTTATCGTGGA	50.0	50.0	57.5	58.2	228 bp	1.0	77.6