Hotspot

ndhH

SSC · 123512-124693 · 1182 bp

Back to Nematolepis

Pi 0.00508

MarkerSeek score 62.00

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 9
pi: 0.00508
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GCACCCACATGATCCCATAG	ACAATTCATGTAAGAATGAATCGAGT	55.0	30.8	58.1	57.5	1305 bp	1.0	40.6
ndhH_p2	GCACCCACATGATCCCATAG	AGAATGAATCGAGTAAAAACCT	55.0	31.8	58.1	53.5	1293 bp	1.0	40.6
ndhH_p3	GGAGTCGAAATAGGGGCAAG	ACAATTCATGTAAGAATGAATCGAGT	55.0	30.8	58.0	57.5	1324 bp	1.0	40.5
ndhH_p4	GGAGTCGAAATAGGGGCAAG	AGAATGAATCGAGTAAAAACCT	55.0	31.8	58.0	53.5	1312 bp	1.0	40.5
ndhH_p5	GGAGTCGAAATAGGGGCAAG	TCATGTAAGAATGAATCGAGT	55.0	33.3	58.0	52.4	1319 bp	1.0	40.5