Hotspot

trnN-GUU-trnR-ACG

IRa · 131313-131928 · 616 bp

Back to Nematolepis

Pi 0.00434

MarkerSeek score 62.00

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 616
variable_sites: 4
pi: 0.00434
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.1	58.1	846-849 bp	1.0	78.8
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.8	58.1	769-772 bp	1.0	78.5
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.2	58.1	756-759 bp	1.0	78.4
trnN-GUU-trnR-ACG_p4	TAGAATCTCCCGCTGTTCCT	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.8	58.1	814-817 bp	1.0	78.4
trnN-GUU-trnR-ACG_p5	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	795-798 bp	1.0	78.2