Hotspot

rpl32-trnL-UAG

SSC · 115000-115593 · 594 bp

Back to Nematolepis

Pi 0.0236
MarkerSeek score 63.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
594
variable_sites
21
pi
0.0236
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACAAATAAATAAGAAAACGTTGGA AGCGTGTCTACCGATTTCAC 25.0 50.0 53.7 58.0 650-651 bp 1.0 42.8
rpl32-trnL-UAG_p2 ACAAATAAATAAGAAAACGTTGGA CAGCGTGTCTACCGATTTCA 25.0 50.0 53.7 58.0 651-652 bp 1.0 42.8
rpl32-trnL-UAG_p3 ACAAATAAATAAGAAAACGTTGGA TCCTAAGAGCAGCGTGTCTA 25.0 50.0 53.7 57.9 660-661 bp 1.0 42.8
rpl32-trnL-UAG_p4 ACAAATAAATAAGAAAACGTTGGA CCTAAGAGCAGCGTGTCTAC 25.0 55.0 53.7 57.8 659-660 bp 1.0 42.8
rpl32-trnL-UAG_p5 ACAAATAAATAAGAAAACGTTGGA CTAAGAGCAGCGTGTCTACC 25.0 55.0 53.7 57.8 658-659 bp 1.0 42.8