Hotspot

petD-rpoA

LSC · 79270-79529 · 260 bp

Back to Nematolepis

Pi 0.0207

MarkerSeek score 60.00

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 8
pi: 0.0207
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CCTTGTGCTTGGGTATTGGA	ACACTTTCGCCTAGAAGATGT	50.0	42.9	57.8	57.0	368-371 bp	1.0	65.0
petD-rpoA_p2	CAGTAGCGACAACCGTCTTT	ACACTTTCGCCTAGAAGATGT	50.0	42.9	58.2	57.0	407-410 bp	1.0	64.7
petD-rpoA_p3	CCTTGTGCTTGGGTATTGGA	TGAACACTTTCGCCTAGAAGA	50.0	42.9	57.8	57.0	371-374 bp	1.0	64.5
petD-rpoA_p4	CAGTAGCGACAACCGTCTTT	TGAACACTTTCGCCTAGAAGA	50.0	42.9	58.2	57.0	410-413 bp	1.0	64.2
petD-rpoA_p5	AGCGACAACCGTCTTTTTGA	ACACTTTCGCCTAGAAGATGT	45.0	42.9	58.3	57.0	403-406 bp	1.0	63.7