Hotspot

psbM-trnD-GUC

LSC · 30569-31160 · 592 bp

Back to Nematolepis

Pi 0.0135

MarkerSeek score 63.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 12
pi: 0.0135
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCAGTAGGAACTAGAACGA	TTTTACTTGCCCGGGATTGT	47.6	45.0	57.9	57.7	739-747 bp	1.0	70.4
psbM-trnD-GUC_p2	AGGCAGTAGGAACTAGAACGA	TACTTGCCCGGGATTGTAGT	47.6	50.0	57.9	58.4	736-744 bp	1.0	69.3
psbM-trnD-GUC_p3	AGCAAAAAGGCAGTAGGAACT	TTTTACTTGCCCGGGATTGT	42.9	45.0	57.8	57.7	746-754 bp	1.0	69.2
psbM-trnD-GUC_p4	AGGCAGTAGGAACTAGAACGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.9	58.5	717-725 bp	1.0	69.0
psbM-trnD-GUC_p5	AGCAAAAAGGCAGTAGGAACT	TACTTGCCCGGGATTGTAGT	42.9	50.0	57.8	58.4	743-751 bp	1.0	68.2