Hotspot

trnR-UCU-atpA

LSC · 10234-10449 · 216 bp

Back to Nematolepis

Pi 0.0216

MarkerSeek score 61.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 216
variable_sites: 7
pi: 0.0216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	CTGAAAGGCGTCCATTGTCT	ACGCTTTCTCCTTCAAGAACA	50.0	42.9	58.2	57.8	311-1668 bp	1.0	71.2
trnR-UCU-atpA_p2	AATGCGCCAAAAATTTCGGA	ACGCTTTCTCCTTCAAGAACA	40.0	42.9	57.6	57.8	337-1667 bp	1.0	69.1
trnR-UCU-atpA_p3	ATGCGCCAAAAATTTCGGAA	ACGCTTTCTCCTTCAAGAACA	40.0	42.9	57.6	57.8	336-1668 bp	1.0	69.1
trnR-UCU-atpA_p4	TGCGCCAAAAATTTCGGAAT	ACGCTTTCTCCTTCAAGAACA	40.0	42.9	57.6	57.8	335-1668 bp	1.0	69.1
trnR-UCU-atpA_p5	CTGAAAGGCGTCCATTGTCT	GAAACCCTTTTGCAAGAAGCT	50.0	42.9	58.2	57.5	349-1706 bp	1.0	69.0