Hotspot

trnS-GCU-trnG-UCC

LSC · 8208-9170 · 963 bp

Back to Nematolepis

Pi 0.038

MarkerSeek score 70.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 963
variable_sites: 49
pi: 0.038
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.6	686-1065 bp	1.0	57.4
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	684-1062 bp	1.0	56.4
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.6	744-1123 bp	1.0	56.0
trnS-GCU-trnG-UCC_p4	CCGACGCTTTAATCCACTCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	686-1064 bp	1.0	55.4
trnS-GCU-trnG-UCC_p5	ACGCTTTAATCCACTCAGCC	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.3	57.6	683-1062 bp	1.0	55.4