Hotspot

trnH-GUG-psbA

LSC · 77-461 · 385 bp

Back to Nematolepis

Pi 0.0365

MarkerSeek score 63.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 21
pi: 0.0365
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	838-843 bp	1.0	81.2
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TCCATTCCACATGTTAGGCG	55.0	50.0	58.1	58.0	885 bp	0.333333	52.0
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TCCATTCCACATGTTAGGCG	50.0	50.0	57.9	58.0	899 bp	0.333333	52.0
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TCCATTCCACATGTTAGGCG	50.0	50.0	57.9	58.0	898 bp	0.333333	52.0
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TCCATTCCACATGTTAGGCG	50.0	50.0	57.9	58.0	900 bp	0.333333	52.0