Hotspot

ndhG-ndhI

SSC · 127567-127945 · 379 bp

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Pi 0.0134

MarkerSeek score 63.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 379
variable_sites: 5
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATACTACTCCTAGGCCCCCT	AAATAGCCACGGGAAAATCC	55.0	45.0	58.1	56.1	511-554 bp	1.0	65.0
ndhG-ndhI_p2	ATACTACTCCTAGGCCCCCT	AAAATAGCCACGGGAAAATCC	55.0	42.9	58.1	56.8	512-555 bp	1.0	63.1
ndhG-ndhI_p3	ACTACTCCTAGGCCCCCTAA	AAATAGCCACGGGAAAATCC	55.0	45.0	58.4	56.1	509-552 bp	1.0	63.1
ndhG-ndhI_p4	ATACTACTCCTAGGCCCCCT	TCAACTAAAAATAGCCACGGGA	55.0	40.9	58.1	57.7	519-562 bp	1.0	62.1
ndhG-ndhI_p5	TACTACTCCTAGGCCCCCTA	AAATAGCCACGGGAAAATCC	55.0	45.0	57.5	56.1	510-553 bp	1.0	61.8