Hotspot

trnF-GAA-ndhJ

LSC · 53248-54007 · 760 bp

Back to Nelumbo

Pi 0.00548

MarkerSeek score 65.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 4
pi: 0.00548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	868-898 bp	1.0	82.2
trnF-GAA-ndhJ_p2	ATAATGCGTCGGGAATGGTC	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	924-954 bp	1.0	81.4
trnF-GAA-ndhJ_p3	GATAATGCGTCGGGAATGGT	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	925-955 bp	1.0	81.4
trnF-GAA-ndhJ_p4	AGGATAATGCGTCGGGAATG	ATCCACGCCTGAAACGTATC	50.0	50.0	57.8	58.1	927-957 bp	1.0	81.0
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	ATCCACGCCTGAAACGTATC	50.0	50.0	58.2	58.1	910-940 bp	1.0	80.8