Pi
0.0117
MarkerSeek score
70.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1081-1133 bp | 1.0 | 83.8 |
| trnT-UGU-trnL-UAA_p2 | GAGTCGAACCGATGACCATC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 1112-1164 bp | 1.0 | 83.5 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1089-1141 bp | 1.0 | 83.2 |
| trnT-UGU-trnL-UAA_p4 | GAGTCGAACCGATGACCATC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 1120-1172 bp | 1.0 | 83.0 |
| trnT-UGU-trnL-UAA_p5 | AAAAGCCAGCTATCGGAGTC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 1127-1179 bp | 1.0 | 82.2 |