Hotspot

psbZ-trnG-UCC

LSC · 40304-40627 · 324 bp

Back to Nelumbo

Pi 0.022

MarkerSeek score 67.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 7
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTCCTGAAGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	475 bp	1.0	73.3
psbZ-trnG-UCC_p2	CCCGTTGTATTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	491 bp	1.0	71.1
psbZ-trnG-UCC_p3	CTTCTCCTGAAGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	478 bp	1.0	71.0
psbZ-trnG-UCC_p4	TCTCCTGAAGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	476 bp	1.0	71.0
psbZ-trnG-UCC_p5	CCCGTTGTATTTGCTTCTCCT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.3	57.8	491 bp	1.0	70.5