Hotspot

trnE-UUC-trnT-GGU

LSC · 34369-35358 · 990 bp

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Pi 0.0193
MarkerSeek score 71.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
990
variable_sites
16
pi
0.0193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TCCATGCCCAACTTCCTTTC GAACCGATGACTTACGCCTT 50.0 50.0 58.1 58.0 984-1147 bp 1.0 84.5
trnE-UUC-trnT-GGU_p2 TCCATGCCCAACTTCCTTTC CCATGGCGTTACTCTACCAC 50.0 55.0 58.1 58.1 963-1126 bp 1.0 84.1
trnE-UUC-trnT-GGU_p3 TCCATGCCCAACTTCCTTTC TGGCGTTACTCTACCACTGA 50.0 50.0 58.1 58.1 960-1123 bp 1.0 83.9
trnE-UUC-trnT-GGU_p4 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 968-1131 bp 1.0 83.9
trnE-UUC-trnT-GGU_p5 TGCCCAACTTCCTTTCGTAG GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 980-1143 bp 1.0 83.7