Hotspot

ycf1

SSC · 132302-136099 · 3798 bp

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Pi 0.00864

MarkerSeek score 48.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3798
variable_sites: 82
pi: 0.00864
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TACCATACTGAAACGGCTGC	GCATTTTTGGGGTTCGATCG	50.0	50.0	58.0	58.1	1237-1238 bp	1.0	81.8
ycf1_p2	TACCATACTGAAACGGCTGC	ACGAACAACATCGAAGCAGA	50.0	45.0	58.0	57.9	780 bp	1.0	81.6
ycf1_p3	ACTGAAACGGCTGCCATTAT	GCATTTTTGGGGTTCGATCG	45.0	50.0	57.9	58.1	1231-1232 bp	1.0	80.8
ycf1_p4	ATACTGAAACGGCTGCCATT	GCATTTTTGGGGTTCGATCG	45.0	50.0	57.9	58.1	1233-1234 bp	1.0	80.8
ycf1_p5	TACCATACTGAAACGGCTGC	TGAACTGGCGTGATTGCTTA	50.0	45.0	58.0	57.8	722 bp	0.8	70.5