Hotspot

rps3-rps19

LSC · 91340-92112 · 773 bp

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Pi 0.00815

MarkerSeek score 61.00

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 14
pi: 0.00815
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	ATAAACTTCCGGGGACATGC	50.0	50.0	57.5	57.9	836-863 bp	1.0	79.6
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	ATAAACTTCCGGGGACATGC	50.0	50.0	58.6	57.9	830-857 bp	1.0	78.8
rps3-rps19_p3	TGGTTGTGCAAACCAAAGAG	ATAAACTTCCGGGGACATGC	45.0	50.0	57.0	57.9	874-901 bp	1.0	75.4
rps3-rps19_p4	TCTGAAACCGAGCGGATTTA	ATAAACTTCCGGGGACATGC	45.0	50.0	57.0	57.9	829-856 bp	1.0	74.9
rps3-rps19_p5	GTCTGAAACCGAGCGGATT	ATAAACTTCCGGGGACATGC	52.6	50.0	57.9	57.9	830-857 bp	1.0	74.1