Hotspot

ndhC-trnV-UAC

LSC · 56543-57625 · 1083 bp

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Pi 0.00332
MarkerSeek score 66.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1083
variable_sites
9
pi
0.00332
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGCCCTTTATTAATCGGGG AAAAGATGCACGTTGGGTCT 50.0 45.0 57.3 58.0 1250-1302 bp 1.0 79.1
ndhC-trnV-UAC_p2 TGGCCCTTTATTAATCGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1179-1231 bp 1.0 77.7
ndhC-trnV-UAC_p3 TGGCCCTTTATTAATCGGGG GATGCACGTTGGGTCTGTAA 50.0 50.0 57.3 58.2 1246-1298 bp 1.0 77.6
ndhC-trnV-UAC_p4 TGGCCCTTTATTAATCGGGG ATGCACGTTGGGTCTGTAAA 50.0 45.0 57.3 57.7 1245-1297 bp 1.0 77.1
ndhC-trnV-UAC_p5 TGGCCCTTTATTAATCGGGG ACGTTGGGTCTGTAAAGCAA 50.0 45.0 57.3 57.7 1241-1293 bp 1.0 76.5