Hotspot

trnT-UGU-trnL-UAA

LSC · 51665-53038 · 1374 bp

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Pi 0.00269
MarkerSeek score 55.20
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1374
variable_sites
9
pi
0.00269
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1434-1456 bp 1.0 79.7
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1442-1464 bp 1.0 79.2
trnT-UGU-trnL-UAA_p3 AAATGCGACGCTCTAACCTC ACCAATTTCGCCATATCCCC 50.0 50.0 58.4 58.0 1406-1428 bp 1.0 78.8
trnT-UGU-trnL-UAA_p4 AAATGCGACGCTCTAACCTC TAGCGTCTACCAATTTCGCC 50.0 50.0 58.4 58.1 1414-1436 bp 1.0 78.3
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 1448-1470 bp 1.0 77.8