Hotspot

trnQ-UUG-psbK

LSC · 7864-8597 · 734 bp

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Pi 0.00259

MarkerSeek score 53.20

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 4
pi: 0.00259
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GATATGCTCTGGGACGGAAG	AGGCTTCAGGTAATTTCACGA	55.0	42.9	57.6	57.3	901-902 bp	1.0	63.3
trnQ-UUG-psbK_p2	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTCACGA	50.0	42.9	58.7	57.3	859-860 bp	1.0	61.5
trnQ-UUG-psbK_p3	GATATGCTCTGGGACGGAAG	AGGCTTCAGGTAATTTCACGAA	55.0	40.9	57.6	58.0	901-902 bp	1.0	60.7
trnQ-UUG-psbK_p4	AATGGATATGCTCTGGGACG	AGGCTTCAGGTAATTTCACGA	50.0	42.9	57.2	57.3	905-906 bp	1.0	60.1
trnQ-UUG-psbK_p5	GAATAGCGGGACCAAAACCC	AGGCTTCAGGTAATTTCACGA	55.0	42.9	58.9	57.3	868-869 bp	1.0	59.7