Hotspot

rps19

IRa · 162137-162415 · 279 bp

Back to Nekemias

Pi 0.00239

MarkerSeek score 25.10

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRa
length_bp: 279
variable_sites: 1
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	GGGTAGAGCCGGATCTAAGT	AATGGCTCGTTCTGAAAGCT	55.0	45.0	58.0	57.8	801-1116 bp	1.0	81.2
rps19_p2	TGGGTAGAGCCGGATCTAAG	AATGGCTCGTTCTGAAAGCT	55.0	45.0	58.0	57.8	802-1117 bp	1.0	81.2
rps19_p3	GAGCCGGATCTAAGTGTTGG	AATGGCTCGTTCTGAAAGCT	55.0	45.0	58.1	57.8	796-1111 bp	1.0	80.8
rps19_p4	GGGTAGAGCCGGATCTAAGT	ATGGCTCGTTCTGAAAGCTC	55.0	50.0	58.0	58.3	800-1115 bp	1.0	80.6
rps19_p5	TGGGTAGAGCCGGATCTAAG	ATGGCTCGTTCTGAAAGCTC	55.0	50.0	58.0	58.3	801-1116 bp	1.0	80.6