Hotspot

psaB

LSC · 41148-43352 · 2205 bp

Back to Nekemias

Pi 0.000907

MarkerSeek score 57.90

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 4
pi: 0.000907
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	GAACCCCTTCTCCATCGAAA	ACAACATGGGCGTTCTTCTT	50.0	45.0	57.2	58.0	2348 bp	1.0	73.7
psaB_p2	AACCCCTTCTCCATCGAAAG	ACAACATGGGCGTTCTTCTT	50.0	45.0	57.2	58.0	2347 bp	1.0	73.7
psaB_p3	ATCGCGGATACAGCACATTA	ACAACATGGGCGTTCTTCTT	45.0	45.0	57.2	58.0	2317 bp	1.0	73.5
psaB_p4	TATCGCGGATACAGCACATT	ACAACATGGGCGTTCTTCTT	45.0	45.0	57.2	58.0	2318 bp	1.0	73.5
psaB_p5	TCGCGGATACAGCACATTAT	ACAACATGGGCGTTCTTCTT	45.0	45.0	57.2	58.0	2316 bp	1.0	73.5