Hotspot

rpl2-rps19

IRa · 161884-162136 · 253 bp

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Pi 0.0152

MarkerSeek score 64.20

Primer available yes

Label rpl2-rps19

Feature Metadata

label_name: rpl2-rps19
feature_type: igs
region: IRa
length_bp: 253
variable_sites: 7
pi: 0.0152
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rps19_p1	CACAACCCCTTGGGGTTATC	TGGTAGATGTCCTGGACCAA	55.0	50.0	58.2	57.7	441-756 bp	1.0	80.4
rpl2-rps19_p2	TTGGGGTTATCCTGCACTTG	TGGTAGATGTCCTGGACCAA	50.0	50.0	57.8	57.7	432-747 bp	1.0	80.0
rpl2-rps19_p3	CACAACCCCTTGGGGTTATC	ATGGTAGATGTCCTGGACCA	55.0	50.0	58.2	57.4	442-757 bp	1.0	78.6
rpl2-rps19_p4	TTGGGGTTATCCTGCACTTG	ATGGTAGATGTCCTGGACCA	50.0	50.0	57.8	57.4	433-748 bp	1.0	78.1
rpl2-rps19_p5	GGGTTATCCTGCACTTGGAA	TGGTAGATGTCCTGGACCAA	50.0	50.0	57.5	57.7	429-744 bp	1.0	77.7