Hotspot

ndhD-ccsA

SSC · 129226-129494 · 269 bp

Back to Nekemias

Pi 0.0206

MarkerSeek score 64.40

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 269
variable_sites: 11
pi: 0.0206
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	CCCGGATTTCGTTCTCTCAT	TGTGGCTTCTATGGGCTTTC	50.0	50.0	57.4	57.9	444-448 bp	1.0	76.8
ndhD-ccsA_p2	ACCCGGATTTCGTTCTCTCA	TGTGGCTTCTATGGGCTTTC	50.0	50.0	58.7	57.9	445-449 bp	1.0	75.8
ndhD-ccsA_p3	CCCGGATTTCGTTCTCTCAT	GTGGCTTCTATGGGCTTTCT	50.0	50.0	57.4	57.6	443-447 bp	1.0	74.5
ndhD-ccsA_p4	ACCCGGATTTCGTTCTCTCA	GTGGCTTCTATGGGCTTTCT	50.0	50.0	58.7	57.6	444-448 bp	1.0	73.4
ndhD-ccsA_p5	CCCGGATTTCGTTCTCTCAT	TTGTGGCTTCTATGGGCTTT	50.0	45.0	57.4	57.4	445-449 bp	1.0	73.0